Diagnosis of intradermal venom allergy

Venom extract and solvent vials are defined as follows,

1 pc: 125 mcg black labeled vial containing powdered venom extract

1 pc: 5 mL pink labeled vial containing solvent

6 pcs: 4.5 mL purple labeled vial containing solvent.

Venoms
  • Apis mellifera (honeybee)
  • Vespula vulgaris / germanica
    (Wasp)

Vial containing venom extract

125 mcg lyophilized venom extract


Reconstitution vials

0.5% phenol saline solution
0.03% Human seum albumin:
5 mL (reconstitution vial)

0.5% phenol saline solution
0.03% Human serum albumin:
4.5 mL (solvent vial)

Preparation scheme

ConcentrationsShelf lifes
Reconstitution100 mcg/mL6 months
1st dilution10 mcg/mL1 month
2nd dilution1 mcg/mL1 week
3rd dilution0.1 mcg/mL1 day
4th dilution0.01 mcg/mL1 day
5th dilution0.001 mcg/mL1 day
6th dilution0.0001 mcg/mL1 day

Reconstitution

Withdraw 1.25 mL of solvent from the light pink labeled solvent vial and transfer to the black labeled vial containing 125 mcg of lyophilized venom extract. A 100 mcg/mL solution is obtained.

1st dilution

Take 0.5 mL from the reconstituted black labeled vial and transfer to the purple labeled 1st dilution vial.

2nd dilution

Take 0.5 mL of the diluted purple labeled vial and transfer to the 2nd dilution vial.

3rd dilution

Take 0.5 mL from the diluted purple labeled vial and transfer to the 3rd dilution vial.

4th dilution

Take 0.5 mL from the diluted purple labeled vial and transfer to the 4th dilution vial.

5th dilution

Take 0.5 mL from the diluted purple labeled vial and transfer to the 5th dilution vial.

6th dilution

Take 0.5 mL of the diluted purple labeled vial and transfer to the 6th dilution vial.

If you need more information about the products and our data sheet is not sufficient, please do not hesitate to contact us:

turkiye@roxall.com

+90 312 240 07 20

Central Office: Mustafa Kemal Mah. 2146 Sk. 14/16, Çankaya/ANKARA

Çankaya Branch: Oğuzlar Mah. 1372 Sk. 3A, Çankaya/ANKARA

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