Diagnosis of Intradermal Venom Allergy

Venom extracts and solvent vials are described as follows:

1 unit: a black-labeled vial containing 125 mcg of powdered venom extract

1 unit: a pink-labeled vial containing 5 mL of solvent

6 units: Vials with purple labels containing 4.5 mL of solvent.

Venomlar
  • Apis mellifera (honeybee)
  • Vespula vulgaris / germanica
    (wild wasp)

Vial containing Venom extract

125 mcg of lyophilized venom extract


Dilution vials

0.5% phenol saline solution
0.03% human serum albumin:
5 mL (dilution vial)

0.5% phenol saline solution
0.03% human serum albumin:
4.5 mL (solvent vial)

Preparation Chart

ConcentrationsRaf lifespans
Dilution100 mcg/mL6 months
1. dilution10 mcg/mL1 month
2. dilution1 mcg/mL1 week
3. dilution0.1 mcg/mL1 day
4. dilution0.01 mcg/mL1 day
5. dilution0.001 mcg/mL1 day
6. dilution0.0001 mcg/mL1 day

Dilution

Draw 1.25 mL of solvent from the light pink-labeled solvent vial and transfer it to the black-labeled vial containing 125 mcg of lyophilized venom extract. This results in a 100 mcg/mL solution.

1. dilution

Take 0.5 mL from the diluted vial labeled “black” and transfer it to the first dilution vial labeled “purple.”

2. dilution

Take 0.5 mL from the vial labeled "purple" and transfer it to the second dilution vial.

3. dilution

Take 0.5 mL from the vial labeled "purple" and transfer it to the third dilution vial.

4. dilution

Take 0.5 mL from the vial labeled "purple" and transfer it to the 4th dilution vial.

5. dilution

Take 0.5 mL from the vial labeled "purple" and transfer it to the 5th dilution vial.

6. dilution

Take 0.5 mL from the vial labeled "purple" and transfer it to the 6th dilution vial.

If you need more information about our products and our data sheet is not sufficient, please don't hesitate to contact us:

turkiye@roxall.com

+90 312 240 07 20

Head Office: Mustafa Kemal Mah. 2146 Sk. 14/16, Çankaya/ANKARA

Çankaya Branch: Oğuzlar Neighborhood, 1372 Street, No. 3A, Çankaya/ANKARA

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